Review



sirna ambion target finder software  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Thermo Fisher sirna ambion target finder software
    Sirna Ambion Target Finder Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+target+finder/pmc10067421-52-24-25
    Average 86 stars, based on 1 article reviews
    sirna ambion target finder software - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    shRNA:

    Article Title: Special AT-rich sequence binding protein 1 promotes tumor growth and metastasis of esophageal squamous cell carcinoma.
    Article Snippet: .. Establishment of stable SATB1-knockdown cell lines Two short-hairpin RNA (shRNA) sequences were designed based on the SATB1 sequence (NM_002971) identified by shRNA Target Finder (Ambion; Life Technologies, Carlsbad, CA, USA): shRNA1 (2566), 5′-GTCCACCT TGTCTTCTCTC-3′; shRNA2 (2364), 5′-AAGGACAATT CCGGTTTAGAG-3′. .. The oligoduplexes were cloned into the pSilencer3.1 vector (Ambion; Life Technologies).

    Article Title: ShRNA-Targeted COMMD7 Suppresses Hepatocellular Carcinoma Growth
    Article Snippet: .. The sequence of short hairpin RNA (shRNA) targeting COMMD7 was designed by using shRNA Target Finder (Ambion, Austin, TX), including Bam H I and Hind III sites at its terminals, and described in . .. Target shRNA was annealed and directly cloned into EGFP reporter plasmid pGenesil-1 (Genesil Biotechnology, Wuhan, China) and subsequently transformed into competent DH5α E. coli (gifted by Dr Y Huang, Chongqing Medical University, Chongqing, China).

    Sequencing:

    Article Title: Special AT-rich sequence binding protein 1 promotes tumor growth and metastasis of esophageal squamous cell carcinoma.
    Article Snippet: .. Establishment of stable SATB1-knockdown cell lines Two short-hairpin RNA (shRNA) sequences were designed based on the SATB1 sequence (NM_002971) identified by shRNA Target Finder (Ambion; Life Technologies, Carlsbad, CA, USA): shRNA1 (2566), 5′-GTCCACCT TGTCTTCTCTC-3′; shRNA2 (2364), 5′-AAGGACAATT CCGGTTTAGAG-3′. .. The oligoduplexes were cloned into the pSilencer3.1 vector (Ambion; Life Technologies).

    Article Title: ShRNA-Targeted COMMD7 Suppresses Hepatocellular Carcinoma Growth
    Article Snippet: .. The sequence of short hairpin RNA (shRNA) targeting COMMD7 was designed by using shRNA Target Finder (Ambion, Austin, TX), including Bam H I and Hind III sites at its terminals, and described in . .. Target shRNA was annealed and directly cloned into EGFP reporter plasmid pGenesil-1 (Genesil Biotechnology, Wuhan, China) and subsequently transformed into competent DH5α E. coli (gifted by Dr Y Huang, Chongqing Medical University, Chongqing, China).



    Similar Products

    sirna ambion target finder software  (Thermo Fisher)
    86
    Thermo Fisher sirna ambion target finder software
    Sirna Ambion Target Finder Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+target+finder/pmc10067421-52-24-25
    Average 86 stars, based on 1 article reviews
    sirna ambion target finder software - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    sirna target finder software  (Thermo Fisher)
    86
    Thermo Fisher sirna target finder software
    (A) Synthetic siRNAs sequences (siRNA1, siRNA2 <t>and</t> <t>siRNA3)</t> targeting the porcine apoE mRNA. (B) Effect of the siRNAs on apoE transcripts levels in cultured porcine granulosa cells. The siRNAs were introduced into the cells by lipofection. Control cells were treated with the lipofection agent alone. Cells were harvested 48 h after treatment and apoE mRNA levels were analyzed by qRT-PCR. Values were normalized to the abundance of GAPDH mRNA. The inhibitory effect of each <t>siRNA</t> was compared to the control group. Values are shown as percent of the control value, as the means ± SEM (n = 3 replicates). Bars that do not share a common superscript are statistically different ( P <0.05).
    Sirna Target Finder Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+target+finder/pmc03667777-90-18-23
    Average 86 stars, based on 1 article reviews
    sirna target finder software - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    shrna target finder  (Thermo Fisher)
    90
    Thermo Fisher shrna target finder
    <t> COMMD7 </t> <t> shRNA </t> sequences and Primer pairs for polymerase chain reaction.
    Shrna Target Finder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+target+finder/pmc03458015-60-13-16
    Average 90 stars, based on 1 article reviews
    shrna target finder - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    sirna target finder  (Thermo Fisher)
    86
    Thermo Fisher sirna target finder
    A . Left panel, immunoblotting of total proteins from INS-1E cells, 72 h after transfection with a negative <t>siRNA</t> (siNeg) <t>or</t> <t>siRNAs</t> against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2). Right panel, quantification of the corresponding Cdk5r2 protein levels. Results are mean ± SEM of three independent experiments. ** P <0.01 versus values of siNeg-transduced INS-1E cells. B . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or with siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (black bars) or not (white bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls. C . Immunoblotting of total proteins from INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (Cyt) or not (Ctrl). The cleavage of caspase-9 and −3, induced by cytokines, is higher in siCdk5r2- than in siNeg-treated cells. D . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), exposed 15 h to control medium (white bars) or palmitate (black bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls.
    Sirna Target Finder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+target+finder/pmc03447792-144-9-12
    Average 86 stars, based on 1 article reviews
    sirna target finder - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    sirna target finder  (InvivoGen)
    86
    InvivoGen sirna target finder
    A . Left panel, immunoblotting of total proteins from INS-1E cells, 72 h after transfection with a negative <t>siRNA</t> (siNeg) <t>or</t> <t>siRNAs</t> against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2). Right panel, quantification of the corresponding Cdk5r2 protein levels. Results are mean ± SEM of three independent experiments. ** P <0.01 versus values of siNeg-transduced INS-1E cells. B . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or with siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (black bars) or not (white bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls. C . Immunoblotting of total proteins from INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (Cyt) or not (Ctrl). The cleavage of caspase-9 and −3, induced by cytokines, is higher in siCdk5r2- than in siNeg-treated cells. D . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), exposed 15 h to control medium (white bars) or palmitate (black bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls.
    Sirna Target Finder, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+target+finder/pmc08643267-38-15-18
    Average 86 stars, based on 1 article reviews
    sirna target finder - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    algorithm sirna target finder  (Thermo Fisher)
    86
    Thermo Fisher algorithm sirna target finder
    A . Left panel, immunoblotting of total proteins from INS-1E cells, 72 h after transfection with a negative <t>siRNA</t> (siNeg) <t>or</t> <t>siRNAs</t> against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2). Right panel, quantification of the corresponding Cdk5r2 protein levels. Results are mean ± SEM of three independent experiments. ** P <0.01 versus values of siNeg-transduced INS-1E cells. B . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or with siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (black bars) or not (white bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls. C . Immunoblotting of total proteins from INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (Cyt) or not (Ctrl). The cleavage of caspase-9 and −3, induced by cytokines, is higher in siCdk5r2- than in siNeg-treated cells. D . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), exposed 15 h to control medium (white bars) or palmitate (black bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls.
    Algorithm Sirna Target Finder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+target+finder/pmc06321724-90-26-32
    Average 86 stars, based on 1 article reviews
    algorithm sirna target finder - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Synthetic siRNAs sequences (siRNA1, siRNA2 and siRNA3) targeting the porcine apoE mRNA. (B) Effect of the siRNAs on apoE transcripts levels in cultured porcine granulosa cells. The siRNAs were introduced into the cells by lipofection. Control cells were treated with the lipofection agent alone. Cells were harvested 48 h after treatment and apoE mRNA levels were analyzed by qRT-PCR. Values were normalized to the abundance of GAPDH mRNA. The inhibitory effect of each siRNA was compared to the control group. Values are shown as percent of the control value, as the means ± SEM (n = 3 replicates). Bars that do not share a common superscript are statistically different ( P <0.05).

    Journal: PLoS ONE

    Article Title: Production of Cloned Pigs with Targeted Attenuation of Gene Expression

    doi: 10.1371/journal.pone.0064613

    Figure Lengend Snippet: (A) Synthetic siRNAs sequences (siRNA1, siRNA2 and siRNA3) targeting the porcine apoE mRNA. (B) Effect of the siRNAs on apoE transcripts levels in cultured porcine granulosa cells. The siRNAs were introduced into the cells by lipofection. Control cells were treated with the lipofection agent alone. Cells were harvested 48 h after treatment and apoE mRNA levels were analyzed by qRT-PCR. Values were normalized to the abundance of GAPDH mRNA. The inhibitory effect of each siRNA was compared to the control group. Values are shown as percent of the control value, as the means ± SEM (n = 3 replicates). Bars that do not share a common superscript are statistically different ( P <0.05).

    Article Snippet: Three synthetic siRNAs (siRNA1, siRNA2 and siRNA3; ) targeting the porcine apoE mRNA (GI:311232) were designed using the siRNA Target Finder software from Ambion (Life Technologies Inc.).

    Techniques: Cell Culture, Quantitative RT-PCR

    COMMD7 shRNA sequences and Primer pairs for polymerase chain reaction.

    Journal: PLoS ONE

    Article Title: ShRNA-Targeted COMMD7 Suppresses Hepatocellular Carcinoma Growth

    doi: 10.1371/journal.pone.0045412

    Figure Lengend Snippet: COMMD7 shRNA sequences and Primer pairs for polymerase chain reaction.

    Article Snippet: The sequence of short hairpin RNA (shRNA) targeting COMMD7 was designed by using shRNA Target Finder (Ambion, Austin, TX), including Bam H I and Hind III sites at its terminals, and described in .

    Techniques: shRNA

    (A) Stably-transfected HepG2 cells on fluorescence microscopy (×200). (B) qRT-PCR quantification and western blotting of COMMD7. (C) MTT assay were performed at indicated days to show the proliferative curve. ** P <0.01 vs sramble shRNA treatment.

    Journal: PLoS ONE

    Article Title: ShRNA-Targeted COMMD7 Suppresses Hepatocellular Carcinoma Growth

    doi: 10.1371/journal.pone.0045412

    Figure Lengend Snippet: (A) Stably-transfected HepG2 cells on fluorescence microscopy (×200). (B) qRT-PCR quantification and western blotting of COMMD7. (C) MTT assay were performed at indicated days to show the proliferative curve. ** P <0.01 vs sramble shRNA treatment.

    Article Snippet: The sequence of short hairpin RNA (shRNA) targeting COMMD7 was designed by using shRNA Target Finder (Ambion, Austin, TX), including Bam H I and Hind III sites at its terminals, and described in .

    Techniques: Stable Transfection, Transfection, Fluorescence, Microscopy, Quantitative RT-PCR, Western Blot, MTT Assay, shRNA

    A . Left panel, immunoblotting of total proteins from INS-1E cells, 72 h after transfection with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2). Right panel, quantification of the corresponding Cdk5r2 protein levels. Results are mean ± SEM of three independent experiments. ** P <0.01 versus values of siNeg-transduced INS-1E cells. B . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or with siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (black bars) or not (white bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls. C . Immunoblotting of total proteins from INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (Cyt) or not (Ctrl). The cleavage of caspase-9 and −3, induced by cytokines, is higher in siCdk5r2- than in siNeg-treated cells. D . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), exposed 15 h to control medium (white bars) or palmitate (black bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls.

    Journal: PLoS ONE

    Article Title: Specific Silencing of the REST Target Genes in Insulin-Secreting Cells Uncovers Their Participation in Beta Cell Survival

    doi: 10.1371/journal.pone.0045844

    Figure Lengend Snippet: A . Left panel, immunoblotting of total proteins from INS-1E cells, 72 h after transfection with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2). Right panel, quantification of the corresponding Cdk5r2 protein levels. Results are mean ± SEM of three independent experiments. ** P <0.01 versus values of siNeg-transduced INS-1E cells. B . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or with siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (black bars) or not (white bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls. C . Immunoblotting of total proteins from INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), treated 24 h with a mix of cytokines (Cyt) or not (Ctrl). The cleavage of caspase-9 and −3, induced by cytokines, is higher in siCdk5r2- than in siNeg-treated cells. D . Quantification of apoptotic nuclei in non-transfected INS-1E cells (NT) and INS-1E cells transfected with a negative siRNA (siNeg) or siRNAs against Cdk5r2 (siCdk5r2#1 and siCdk5r2#2), exposed 15 h to control medium (white bars) or palmitate (black bars). Results are mean ± SEM of six independent experiments. ** P <0.01 versus controls.

    Article Snippet: Specific siRNAs against rat Cdk5r2 were selected using the siRNA Target Finder (Ambion, Austin, TX, USA).

    Techniques: Western Blot, Transfection